RAPID AMPLIFICATION OF RM-Yplex ASSAY
نویسنده
چکیده
A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was developed. Platinum® Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction in approximately 2.5 hours . In order to shorten the process with reliable results, several DNA polymerases were tested with the multiplex. Phusion® Flash High Fidelity and Platinum® Taq DNA polymerases were investigated for conducting RM-Yplex assay at various PCR cycling conditions. Rapid, robust and efficient amplification of all markers within the multiplex was achieved. The amplification time was reduced from 2.5 hours to less than 28 minutes using Verti® PCR machine.
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